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Abstract The cerebellum regulates nonmotor behavior, but the routes of influence are not well characterized. Here we report a necessary role for the posterior cerebellum in guiding a reversal learning task through a network of diencephalic and neocortical structures, and in flexibility of free behavior. After chemogenetic inhibition of lobule VI vermis or hemispheric crus I Purkinje cells, mice could learn a water Y-maze but were impaired in ability to reverse their initial choice. To map targets of perturbation, we imaged c-Fos activation in cleared whole brains using light-sheet microscopy. Reversal learning activated diencephalic and associative neocortical regions. Distinctive subsets of structures were altered by perturbation of lobule VI (including thalamus and habenula) and crus I (including hypothalamus and prelimbic/orbital cortex), and both perturbations influenced anterior cingulate and infralimbic cortex. To identify functional networks, we used correlated variation in c-Fos activation within each group. Lobule VI inactivation weakened within-thalamus correlations, while crus I inactivation divided neocortical activity into sensorimotor and associative subnetworks. In both groups, high-throughput automated analysis of whole-body movement revealed deficiencies in across-day behavioral habituation to an open-field environment. Taken together, these experiments reveal brainwide systems for cerebellar influence that affect multiple flexible responses. 

The structural complexity of oyster reef canopy plays a major role in promoting biodiversity, balancing the sediment budget, and modulating hydrodynamics in estuarine systems. While oyster canopy structure is both spatially and temporally heterogeneous, oyster canopies are generally characterized using simple first-order quantities, like oyster density, which may lack the ability to sufficiently parameterize reef roughness. In this study, a novel laser scan approach was used to map the surface of intact reference and restored reefs (restoration age: 1 – 4 years) during low tide, when the oyster canopy was fully exposed. Measurements were used to estimate hydrodynamically-relevant roughness characteristics over the entire reef surface (>140 m2; 0.50 m resolution), providing estimates of the canopy height (hc), standard deviation (σ_c), rugosity index (R), and fractal dimension (D). Average canopy heights ranged from 3.6 – 4.9 cm, with canopy height standard deviations between 1.4 and 2.0 cm. Mean rugosity indices and fractal dimensions were relatively low on the youngest (1 year) restored reef (R=1.21; D=2.67), with substantial increases observed for more mature reef canopies (4 years: R=1.51; D=2.71). Structural complexity was consistently greater on reef margins than in reef interiors. Increases in complexity were linked to restoration age, with older reefs exhibiting more complex oyster canopies. The highest fractal dimension was observed on the intact reference reef, highlighting the importance of sustained reef growth for maintaining higher-order structural complexity. Results provide spatially explicit surface roughness characterizations for healthy, intertidal oyster reefs, with applications in both restoration science and natural and nature-based feature design. 

Creating burrows through natural soils and sediments is a problem that evolution has solved numerous times, yet burrowing locomotion is challenging for biomimetic robots. As for every type of locomotion, forward thrust must overcome resistance forces. In burrowing, these forces will depend on the sediment mechanical properties that can vary with grain size and packing density, water saturation, organic matter and depth. The burrower typically cannot change these environmental properties, but can employ common strategies to move through a range of sediments. Here we propose four challenges for burrowers to solve. First, the burrower has to create space in a solid substrate, overcoming resistance by e.g., excavation, fracture, compression, or fluidization. Second, the burrower needs to locomote into the confined space . A compliant body helps fit into the possibly irregular space, but reaching the new space requires non-rigid kinematics such as longitudinal extension through peristalsis, unbending, or eversion. Third, to generate the required thrust to overcome resistance, the burrower needs to anchor within the burrow . Anchoring can be achieved through anisotropic friction or radial expansion, or both. Fourth, the burrower must sense and navigate to adapt the burrow shape to avoid or access different parts of the environment. Our hope is that by breaking the complexity of burrowing into these component challenges, engineers will be better able to learn from biology, since animal performance tends to exceed that of their robotic counterparts. Since body size strongly affects space creation, scaling may be a limiting factor for burrowing robotics, which are typically built at larger scales. Small robots are becoming increasingly feasible, and larger robots with non-biologically-inspired anteriors (or that traverse pre-existing tunnels) can benefit from a deeper understanding of the breadth of biological solutions in current literature and to be explored by continued research. 

In 2021, people of Hispanic and Latinx origin made up 6% of the atmospheric and Earth sciences workforce of the United States, yet they represent 20% of the population. Motivated by this disparity in Hispanic and Latinx representation in the atmospheric and Earth science workforce, this manuscript documents the lack of representation through existing limited demographic data. The analysis presents a clear gap in participation by Hispanic and Latinx people in academic settings, with a widening gap through each education and career stage. Several factors and challenges impacting the representation disparity include the lack of funding for and collaboration with Hispanic-serving institutions, limited opportunities due to immigration status, and limited support for international research collaborations. We highlight the need for actionable steps to address the lack of representation and provide targeted recommendations to federal funding agencies, educational institutions, faculty, and potential employers. While we wait for systemic cultural change from our scientific institutions, grassroots initiatives like those proudly led by the AMS Committee for Hispanic and Latinx Advancement will emerge to address the needs of the Hispanic and Latinx scientific and broader community. We briefly highlight some of those achievements. Lasting cultural change can only happen if our leaders areactiveallies in the creation of a more diverse, equitable, and inclusive future. Alongside our active allies we will continue to champion for change in our weather, water, and climate enterprise. 

Bacteria that live inside the cells of insect hosts (endosymbionts) can alter the reproduction of their hosts, including the killing of male offspring (male killing, MK). MK has only been described in a few insects, but this may reflect challenges in detecting MK rather than its rarity. Here, we identify MK Wolbachia at a low frequency (around 4%) in natural populations of Drosophila pseudotakahashii . MK Wolbachia had a stable density and maternal transmission during laboratory culture, but the MK phenotype which manifested mainly at the larval stage was lost rapidly. MK Wolbachia occurred alongside a second Wolbachia strain expressing a different reproductive manipulation, cytoplasmic incompatibility (CI). A genomic analysis highlighted Wolbachia regions diverged between the 2 strains involving 17 genes, and homologs of the wmk and cif genes implicated in MK and CI were identified in the Wolbachia assembly. Doubly infected males induced CI with uninfected females but not females singly infected with CI-causing Wolbachia . A rapidly spreading dominant nuclear suppressor genetic element affecting MK was identified through backcrossing and subsequent analysis with ddRAD SNPs of the D . pseudotakahashii genome. These findings highlight the complexity of nuclear and microbial components affecting MK endosymbiont detection and dynamics in populations and the challenges of making connections between endosymbionts and the host phenotypes affected by them.